影响猪免疫性状的单倍型关联分析

doi:10.11843/j.issn.0366-6964.2013.06.003

Abstract

Abstract:

The aim of the present study was to narrow down the range of previous genome wide association study (GWAS) , and to provide valid candidate genes for the large-scale group validation. Seven immune traits including four blood parameters(hematocrit (HCT), hemoglobin(HGB), mean corpuscular hemoglobin (MCH), mean cell volume (MCV)) and three T lymphocyte subpopulation parameters(CD4+CD8-CD3+, CD4-CD8+CD3+ and CD4+CD8-CD3+/CD4-CD8+CD3+)were measured in a porcine intercross population(Large White×Min zhu). Six haplotype blocks were built using the significant SNPs tested in the previous GWAS. Association studies between the haplotype blocks and the immune traits were performed. The result showed that the three blocks on SSC7 were significantly associated with the three T lymphocyte subpopulation parameters, the block7-3 on SSC7 was significantly associated with HGB and HCT, and the three blocks on SSC8 were significantly associated with MCH and MCV. The six blocks contained five genes ABCF1, PPP1R10, GRM4, ALB and AFP. Combination of these results, the five genes could be used as potential candidate genes for the study on immune traits.

CLC Number:

S828
S813.3

LUO Wei-zhen, CHEN Shao-kang, ZHANG Long-chao, CHENG Du-xue, YAN Hua, LI Yong, WANG Li-gang, SONG Xin, MA Xiao-jun, LIU Xin, LI Wen, LIANG Jing,ZHAO Ke-bin, WANG Li-xian. Haplotype Association Study for Immune Traits in Swine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(6): 843-852.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2013.06.003

https://www.xmsyxb.com/EN/Y2013/V44/I6/843

References

MÜLLER M, BREM G. Disease resistance in farm animals ［J］.Experientia, 1991, 47(9):923-934.

JIANG L, LIU J F, SUN D X, et al. Genome wide association studies for milk production traits in chinese holstein population ［J］.PLoS ONE, 2010, 5(10): e13661.

SAHANA G, GULDBRANDTSEN B, BENDIXEN C, et al. Genome-wide association mapping for female fertility traits in Danish and Swedish Holstein cattle ［J］. Anim Genet, 2010, 41(6):579-588.

BOLORMAA S, HAYES B J, SAVIN K, et al. Genome-wide association studies for feedlot and growth traits in cattle ［J］. Anim Sci, 2011, 89(6):1684-1697.

GU X, FENG C, MA L, et al.Genome-wide association study of body weight in chicken F2 resource population ［J］.PLoS ONE, 2011, 6(7): e21872.

FAN B, ONTERU S K, DU Z Q, et al. Genome-wide association study identifies loci for body composition and structural soundness traits in pigs ［J］.PLoS ONE, 2011, 6:e14726.

LUO W Z, CHENG D X, CHEN S K, et al. Genome-wide association study of meat quality traits in a Large White × Minzhu F2 resourse population ［J］. Int J Biol Sci, 2012, 8(4):561-579.

LUO W Z, CHEN S K, CHENG D X, et al. Genome-wide association study for porcine haematological parameters in a Large White × Minzhu F2 resourse population ［J］. Int J Biol Sci, 2012, 8(6):870-881.

BARRETT J C, FRY B, MALLER J, et al. HAPLOVIEW: analysis and visualization of LD and haplotype maps ［J］.Bioinformatics, 2005, 21:263-265.

SCHAID D J, ROWLAND C M, TINES D E, et al. Score tests for association between traits and haplotypes when linkage phase is ambiguous ［J］.Am J Hum Genet, 2002, 70(2):425-434.

杨汉春. 动物免疫学［M］.北京：中国农业大学出版社,2003.

MORRIS R W, KAPLAN N L. On the advantage of haplotype analysis in the presence of multiple disease susceptibility alleles ［J］. Genet Epidemiol, 2002, 23(3):221-233.

MARTIN E R, MONKS S A, WARREN L L, et al. A test for linkage and association in general pedigrees: the pedigree disequilibrium test ［J］. Am J Hum Genet，2000, 67(1):146-154.

PAYTUBI S, MORRICE N A, BOUDEAU J, et al. The N-terminal region of ABC50 interacts with eukaryotic initiation factor eIF2 and is a target for regulatory phosphorylation by CK2 ［J］.Biochem J, 2008, 409(1):223-231.

PAYTUBI S, WANG X, LAM Y W, et al. ABC50 promotes translation initiation in mammalian cells ［J］.J Biol Chem, 2009, 284(36):24061-24073.

KREIVI J P, TRINKLE-MULCAHY L, LYON C E, et al. Purification and characterisation of p99, a nuclear modulator of protein phosphatase 1 activity［J］.FEBS Lett, 1997, 420(1): 57-62.

ALLEN P B, KWON Y G, NAIRN A C, et al. Isolation and characterization of PNUTS, a putative protein phosphatase 1 nuclear targeting subunit ［J］.Biol Chem, 1998, 273: 4089-4095.

WALRAT H, JESSICA C. Characterization of the genes at the Epi-2 locus and their potential roles in Neurofibromatosis 1-dependent myeloid leukemia ［M］.University of Florida, 2005.

MUHLE H, VON SPICZAK S, GAUS V, et al. Role of GRM4 in idiopathic generalized epilepsies analysed by genetic association and sequence analysis ［J］.Epilepsy Res, 2010, 89(2-3):319-326.

韦麒，苏明华，江建宁，等血清白蛋白、球蛋白与慢性乙型病毒性肝炎病理的相关性研究［J］.广西医科大学学报，2011，28(3):385-387.

陈叶青，周国华，周红宇，等肝硬化患者血清球蛋白与 Child Turcotte Pugh分级的相关性研究［J］.疑难病杂志，2008，7(2):95-96.

朱云松，杨大明，姚登福，等原发性肝癌外周血白蛋白基因片段扩增的临床意义［J］. 南通大学学报（医学版），2008，28(4):261-263.

董天皞 ShRNA介导的AFP基因沉默对肝癌细胞增殖的影响［D］.天津: 南开大学，2006.

[1]	NIU Naiqi, ZHAO Runze, ZONG Wencheng, LIU Xiance, LIU Hai, SHI Guohua, JING Xitao, ZHANG Longchao. Association of Polymorphisms of GREB1L and MIB1 Genes with Rib Number and Carcass Traits in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 79-86.
[2]	ZHU Xueli, ZHANG Longchao, WANG Lixian, PU Lei, LIU Xin. Association Analysis of AQP9 and RPS10 Gene Polymorphisms with Backfat Thickness in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 87-98.
[3]	SHI Shengjie, WANG Liguang, GAO Lei, CAI Chuanjiang, HE Weixian, CHU Guiyan. Effect of miR-24-3p on Estradiol Synthesis in Porcine Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 169-178.
[4]	YANG Kai, LU Zhuoda, HE Jian, ZHANG Ruiqi, WANG Suqing, LI Kebiao, ZHAO Yunxiang, ZHU Xiaoping, GUO Jinbiao. The Population Effect of Commercial Pigs on the Accuracy of Genomic Selection for Carcass Traits in Duroc Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4943-4951.
[5]	LI Chao, ZHAO Xueyan, WANG Yongjun, WANG Yanping, REN Yifan, LI Jingxuan, WANG Huaizhong, WANG Jiying, SONG Qinye. Analysis on the Structural Composition and Function of Bacterial Microbiota of Caecum and Colon in Laiwu and DLY pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5033-5045.
[6]	XU Tingting, QI Fenfang, HUANG Shihui, NIU Xi, LI Sheng, RAN Xueqin, WANG Jiafu, XIE Jian. Study of the Polymorphisms of Structural Variation and the Expression of MAP3K4 gene in Xiang Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5046-5055.
[7]	XUE Hongyan, YANG Mengyu, YANG Huan, DONG Lijun, CAI Xiaqing, ZHAO Zemin, WANG Xianzhong. The Role of ALOX15B-JNK in Heat Stress-induced Oxidative Stress and Apoptosis of Sertoli Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5056-5065.
[8]	HU Ziping, WANG Ligang, ZONG Wencheng, HOU Renda, SU Yanfang, NIU Naiqi, WANG Lixian, WANG Yuan, ZHNAG Longchao. Genetic Structure Analysis of Jianbai Xiang Pig Population Based on Genomic SNP and ROH [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4117-4125.
[9]	JI Zhengyu, NI Mengru, ZHANG Zhaobo, ZHAO Gan, HUANG Zan, LI Pinghua, HUANG Ruihua, HOU Liming. Research on Adipogenic Capacity and Gene Expression Pattern of in Vitro FAPs Cells Derived from Longissimus Dorsi Muscle of Suhuai Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4126-4142.
[10]	BI Huan, QIN Hai, YUAN Wei, ZHANG Yudan, ZHANG Yiyu, CHEN Wei. Effect of Knocking down TYRP1 Gene on Melanin Production in the Xiang Pig Epidermal Melanocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4143-4153.
[11]	LIU Pan, LI Ruiqi, TAN Zhankun, WANG Yifei, CHEN Xiaochen, HE Weixian, DU Renrang, MA Jian, CHU Guiyan, CAI Chuanjiang. Effects of High Fiber Diet on Growth Performance, Meat Quality and Intestinal Microbiome of Growing-finishing Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4247-4259.
[12]	LI Pinghui, PU Guang, WANG Zhongyu, ZHOU Wuduo, NIU Peipei, WU Chengwu, HOU Liming, HUANG Ruihua, LI Pinghua. Effect of Dietary Fiber Level on Blood and Intestinal Immune Indexes of Meishan Pigs and Preliminary Analysis of Its Mechanism [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4260-4277.
[13]	WANG Hui, FENG Baoliang, WU Dan, XIANG Guangming, WANG Nan, MU Yulian, LI Kui, LIU Zhiguo. Research Progress of CD163 Gene and Disease-Resistant Breeding on Porcine Reproductive and Respiratory Syndrome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3127-3138.
[14]	ZHANG Wanfeng, ZHAO Tianzhi, LI Jiao, YOU Ziwei, YANG Yang, CAI Chunbo, GAO Pengfei, CAO Guoqing, GUO Xiaohong, LI Bugao. Study on NR2F2 Gene Regulating Proliferation and Apoptosis of Porcine PK15 Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3242-3251.
[15]	LIAN Yuju, ZHANG Zhiyuan, LIAO Xiaobo, WEI Hongjiang, YIN Yulong, LIU Mei. Breeding Methods and Application Progress of Medical Miniature Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2667-2682.

Haplotype Association Study for Immune Traits in Swine

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments